Separation of lymphocytes from peripheral blood: improvement of yields using buffy coat preparations.
نویسندگان
چکیده
منابع مشابه
Micromethod for rapid separation of lymphocytes from peripheral blood.
The separation of lymphocytes from the other blood cells is based on the differences in their specific gravity. Centrifugation of whole blood against an isopaque-ficoll gradient results in the formation of three separate layers: the top one contains the slowly sedimenting cells (lymphocytes, platelets and some monocytes); the inter mediate one, the granulocytes; the bottom layer contains the e...
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11 Confirmative diagnosis of Visceral Leishmaniasis (VL) is still being a challenge at the primary 12 health care facilities in most of the rural endemic areas in the Indian sub-continent. Conventional 13 methods for parasitological confirmation are risky and require skilled personnel hence, 14 unreachable to the poor people in the endemic region. Buffy coat smear microscopy, as a 15 minimally ...
متن کاملEvaluation of buffy coat 16S rRNA PCR, buffy coat culture and whole blood PCR for detection of bacteraemia.
The use of Gram type-specific PCR on buffy coat from clinical specimens for the detection of bacteraemia was evaluated for the first time using whole blood culture as the gold standard. In addition, the established buffy coat culture and whole blood PCR were also compared. Gram-positive bacteria belonging to six species and Gram-negative bacteria from 10 species were isolated and identified by ...
متن کاملPeripheral blood buffy coat smear: a promising tool for diagnosis of visceral leishmaniasis.
Confirmative diagnosis of visceral leishmaniasis (VL) is still a challenge at the primary health care facilities in most of the rural areas of endemicity in the Indian subcontinent. Conventional methods for parasitological confirmation are risky and require skilled personnel, and hence they are unavailable to the poor people in the regions of endemicity. Buffy coat smear microscopy, as a minima...
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A rapid test for diagnosis of malaria based on acridine orange staining of centrifuged blood samples in a microhaematocrit tube (QBC) was compared with Leishman stained thin peripheral blood smear in 287 samples. Malaria was diagnosed in 44 patients by Leishman staining technique and in 65 patients by QBC method. The QBC method allowed detection of an additional 21 cases. Thus the prevalence ra...
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ژورنال
عنوان ژورنال: Journal of Clinical Pathology
سال: 1985
ISSN: 0021-9746
DOI: 10.1136/jcp.38.12.1405